Implementation of super-resolution cryo-fluorescence microscopy under Abbe's diffraction limit and its correlative combination with scanning electron microscopy as an innovative tool in biomedical research and diagnostics

While diffraction effects lead to a resolution limit in ordinary light microscopy (Abbe Limit), the super-resolution cryo-fluorescence microscopy explored here enables a much higher resolution. The additional combination with cryo-scanning electron microscopy enables molecules to be imaged in their original cellular context and allows simultaneous analysis of the molecular organization and ultrastructural architecture of intact cells. This is expected to provide a deeper understanding of how diseases and organisms function.